| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.137159 |
| Chromosome: | chromosome 17 |
| Location: | 191905 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g697500 | P25 Alpha Domain and EF Hand Protein; (1 of 2) PTHR12932:SF9 - TPPP FAMILY PROTEIN CG45057 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTGATGTGGTGAGTGGGTGGCTGCACG |
| Internal bar code: | GCCACCGGGCCTCTGTTCCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 968 |
| LEAP-Seq percent confirming: | 99.2328 |
| LEAP-Seq n confirming: | 14746 |
| LEAP-Seq n nonconfirming: | 114 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTACCACTCCAAACGGG |
| Suggested primer 2: | GGATGTGCTTGTGTGGTGTC |