| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.137205 |
| Chromosome: | chromosome 10 |
| Location: | 128141 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418600 | RLS3 | RegA/Rls-like protein; (1 of 10) IPR010919 - SAND domain-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGCGCTAGGAGATCGGATGGTGTGATTT |
| Internal bar code: | CGGCCCGCGGCTCGGGCTCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 147 |
| LEAP-Seq percent confirming: | 95.1497 |
| LEAP-Seq n confirming: | 1589 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGAGCAAGCTGGTAGGTC |
| Suggested primer 2: | CCTATCTAGCCTGCCTGCAC |