Insertion junction: LMJ.RY0402.137212_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.137212
Chromosome:chromosome 2
Location:1990314
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g088350 SNR3 (1 of 6) PF09335 - SNARE associated Golgi protein (SNARE_assoc); SNARE associated Golgi protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGAGAGTCACCTGGGCCCACAAACTTCA
Internal bar code:CGACGCGTTGTGTCATTAGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:974
LEAP-Seq percent confirming:99.4144
LEAP-Seq n confirming:12223
LEAP-Seq n nonconfirming:72
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCATCACCGTCTCTCCAT
Suggested primer 2:CCTTTGTCACGTGAACCCTT