| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137235 |
| Chromosome: | chromosome 10 |
| Location: | 3064790 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441550 | MAM3B | (1 of 5) K16302 - metal transporter CNNM (CNNM); Protein putatively involved in mitochondrial biogenesis | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGTGCAGGTGGGTGCGGGGGCAGGTGGG |
| Internal bar code: | AACGTCGGCGTCCGAGCCGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 821 |
| LEAP-Seq percent confirming: | 98.6978 |
| LEAP-Seq n confirming: | 3259 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGCTTGAGTTCGGGTAT |
| Suggested primer 2: | TGAGCTCTCATCACCTGCAA |