| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137235 |
| Chromosome: | chromosome 10 |
| Location: | 3064801 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441550 | MAM3B | (1 of 5) K16302 - metal transporter CNNM (CNNM); Protein putatively involved in mitochondrial biogenesis | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCCTCCAGAGTGATGATGCCCACAGGCA |
| Internal bar code: | ATCAAGGGGGTGGTTCGTTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 625 |
| LEAP-Seq percent confirming: | 99.7617 |
| LEAP-Seq n confirming: | 5443 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGCTCTCATCACCTGCAA |
| Suggested primer 2: | CCATGCTTGAGTTCGGGTAT |