Insertion junction: LMJ.RY0402.137246_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.137246
Chromosome:chromosome 2
Location:1689738
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g085850 ARS6 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGACTGGTCTTGCATGCAAACAACACTG
Internal bar code:GCCCGATCGGGAAGGTCAAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:938
LEAP-Seq percent confirming:97.6103
LEAP-Seq n confirming:64046
LEAP-Seq n nonconfirming:1568
LEAP-Seq n unique pos:143

Suggested primers for confirmation by PCR

Suggested primer 1:TCTTGTGCACCTACGGTTTG
Suggested primer 2:CGTGAATCCGTTGAGGAACT