| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137266 |
| Chromosome: | chromosome 14 |
| Location: | 330778 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610050 | (1 of 1) K15180 - negative elongation factor B (COBRA1, NELFB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGAAGGAGGTTGCGAGTTGTTGGCGCCA |
| Internal bar code: | GGCACGTCGTCGTAATGTATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 247 |
| LEAP-Seq percent confirming: | 99.4887 |
| LEAP-Seq n confirming: | 1362 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTAAACCGAACCCAACGC |
| Suggested primer 2: | ACGTTGAGGATCGGTGGTAG |