| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.137267 |
| Chromosome: | chromosome 9 |
| Location: | 5945314 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGACGGTTCGCCTGCACACATCCGGACAC |
| Internal bar code: | TTACGTCGGGATCCAAGTACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 957 |
| LEAP-Seq percent confirming: | 99.5456 |
| LEAP-Seq n confirming: | 14677 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGCGTTGTTTTATCAGGG |
| Suggested primer 2: | ACAGTGGGTCGGGTAGACAG |