| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.137278 |
| Chromosome: | chromosome 2 |
| Location: | 599981 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077800 | CSE13,FAP310 | Flagellar Associated Protein 310 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCTCATGCAGTCACCTGGTCGGCAACC |
| Internal bar code: | ATGTGACCGATGTTTCTCTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 949 |
| LEAP-Seq percent confirming: | 98.773 |
| LEAP-Seq n confirming: | 6118 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGCTAGGCACCTGGTTTG |
| Suggested primer 2: | GTGTCCATCTACACGCCCTT |