| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.137297 |
| Chromosome: | chromosome 1 |
| Location: | 6238608 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044600 | (1 of 2) PF17047 - Synaptotagmin-like mitochondrial-lipid-binding domain (SMP_LBD) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTTTGGACACCGCTTGAGGCCGTGAGGG |
| Internal bar code: | CCGCCAGCTCGAAGATGTTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 392 |
| LEAP-Seq percent confirming: | 99.8477 |
| LEAP-Seq n confirming: | 13112 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCGATGGTTGATAGCCTC |
| Suggested primer 2: | GAGACTTGACGTGATTGCGA |