| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.137309 |
| Chromosome: | chromosome 6 |
| Location: | 7847362 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g302950 | (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGTGGGCGGGGGAGAACTGCATTACCTG |
| Internal bar code: | AGGCAAAAGTTGACTAAGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 144 |
| LEAP-Seq percent confirming: | 99.0641 |
| LEAP-Seq n confirming: | 2858 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGCTTCACCTCTAGTCCG |
| Suggested primer 2: | AACAAAACGTTCCCTATGCG |