| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137309 |
| Chromosome: | chromosome 10 |
| Location: | 3288495 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443350 | (1 of 1) PTHR13258//PTHR13258:SF0 - UNCHARACTERIZED // COILED-COIL DOMAIN-CONTAINING PROTEIN 132 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATAAAATACACAGACTCGCAAATACACGG |
| Internal bar code: | GGGAAGTGACGGCTCAGATGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 879 |
| LEAP-Seq percent confirming: | 99.5091 |
| LEAP-Seq n confirming: | 1419 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGATCCGAACACAACATGG |
| Suggested primer 2: | AACATGTCCCTGGACCTCAG |