Insertion junction: LMJ.RY0402.137337_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.137337
Chromosome:chromosome 9
Location:3705089
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g390912 (1 of 54) IPR001680//IPR017986 - WD40 repeat // WD40-repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCAGAGACGGAGGCGGCGCTTCCACGCA
Internal bar code:GCGTGAATTGGACCACAGTCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:770
LEAP-Seq percent confirming:96.2441
LEAP-Seq n confirming:7995
LEAP-Seq n nonconfirming:312
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:TTCTGAACCGTACTCCCACC
Suggested primer 2:GTGTGATGTGGTGGAGCAAC