Insertion junction: LMJ.RY0402.137357_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.137357
Chromosome:chromosome 17
Location:6882279
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g745647 (1 of 1) PF09350 - Domain of unknown function (DUF1992) (DUF1992) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTAGCGGGGCCGCATTCGGGACCACCCCC
Internal bar code:CTTGACACTAAGACGAATGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:476
LEAP-Seq percent confirming:13.0416
LEAP-Seq n confirming:885
LEAP-Seq n nonconfirming:5901
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GAACAGACTGACCCCATGCT
Suggested primer 2:CCATGTTACTGAGGCCAGGT