| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137357 |
| Chromosome: | chromosome 17 |
| Location: | 6882304 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g745647 | (1 of 1) PF09350 - Domain of unknown function (DUF1992) (DUF1992) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCAGACTCCACACGGGGGACACGCCCG |
| Internal bar code: | GCAAAGTCGTCACAGTCGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 802 |
| LEAP-Seq percent confirming: | 98.6116 |
| LEAP-Seq n confirming: | 5611 |
| LEAP-Seq n nonconfirming: | 79 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACAGACTGACCCCATGCT |
| Suggested primer 2: | CCATGTTACTGAGGCCAGGT |