Insertion junction: LMJ.RY0402.137363_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.137363
Chromosome:chromosome 2
Location:779788
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g078804 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCGGCGGCAGTACACCTGAGCTCACATC
Internal bar code:TGGTGACGACGCACGTGCAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:448
LEAP-Seq percent confirming:99.4823
LEAP-Seq n confirming:8840
LEAP-Seq n nonconfirming:46
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGGGGCAGTAGGTAGTGGAG
Suggested primer 2:CACCGACGAAGCTCTCTACC