| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137372 |
| Chromosome: | chromosome 9 |
| Location: | 5884719 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402441 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAGCAAGGGGGTTGTGCGTGTGCGTTAC |
| Internal bar code: | CCGAAAGTGTACGGCAATAAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 559 |
| LEAP-Seq percent confirming: | 98.893 |
| LEAP-Seq n confirming: | 10095 |
| LEAP-Seq n nonconfirming: | 113 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGAACAAGGAAGCTCAC |
| Suggested primer 2: | TGCTATCCTTCTCCCTGCAT |