| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137377 |
| Chromosome: | chromosome 12 |
| Location: | 6120800 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535900 | ARL1 | ARF-like GTPase; (1 of 1) K07942 - ADP-ribosylation factor-like protein 1 (ARL1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGTGTCCCGCACCCGCGGGTGCCCGCTG |
| Internal bar code: | GTGTTGGTACAATTATTTCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 888 |
| LEAP-Seq percent confirming: | 99.6504 |
| LEAP-Seq n confirming: | 1140 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGAGAGCGTGTGGAAAG |
| Suggested primer 2: | GAGTGCAGGGGAAGTAGCAG |