| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.137392 |
| Chromosome: | chromosome 14 |
| Location: | 1707596 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619400 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACAGCGCTCAGCGGCGGGGCGGTCTTC |
| Internal bar code: | ACGGATCGAGACGACCTCACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 359 |
| LEAP-Seq percent confirming: | 98.9734 |
| LEAP-Seq n confirming: | 5881 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATAGTGTTGGCGAGAGCA |
| Suggested primer 2: | GCTGCCCGTAGTAGTCCAAG |