| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137403 |
| Chromosome: | chromosome 10 |
| Location: | 5779871 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461319 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGCGCTGGGCACCAGGTAGCTGCTGCCG |
| Internal bar code: | CGGCTCAGATCCGTTGATCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 442 |
| LEAP-Seq percent confirming: | 95.183 |
| LEAP-Seq n confirming: | 494 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCGTCTCCTGAAGGTAGT |
| Suggested primer 2: | GCAGAGGACGCAGGTTTTAG |