| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137409 |
| Chromosome: | chromosome 17 |
| Location: | 3290316 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723100 | (1 of 10) PTHR31867:SF1 - EXPANSIN-A1-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGCTAATCAAGCGCCCAGACCGGATGCT |
| Internal bar code: | CTGGCGCCTCGACACACCTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1046 |
| LEAP-Seq percent confirming: | 99.3065 |
| LEAP-Seq n confirming: | 7017 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAATAAGGAGAGCGCAAC |
| Suggested primer 2: | TAGCTCAAACGTTGTGCCTG |