| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137422 |
| Chromosome: | chromosome 12 |
| Location: | 4704982 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523500 | DER1 | DERLIN-1 involved in ER-associated degradation system; (1 of 2) K13989 - Derlin-2/3 (DERL2_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGAGGGAGTCGGCCATGAAGAACATGGG |
| Internal bar code: | GTGAGCAGACCGCGTCATTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 190 |
| LEAP-Seq percent confirming: | 99.8569 |
| LEAP-Seq n confirming: | 698 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAAGAATCCACCCCTACA |
| Suggested primer 2: | CCAGCAGGTTCTTGAGGAAG |