| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.137423 |
| Chromosome: | chromosome 16 |
| Location: | 2886756 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g663650 | TRS33 | Component of TRAPP complex | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTGCACTGGATTGCAGCCCTTGTTGCA |
| Internal bar code: | GAGCTCGCTGCTGGTGGCGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 935 |
| LEAP-Seq percent confirming: | 98.8182 |
| LEAP-Seq n confirming: | 2174 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTTTAGTTTGGGCTGG |
| Suggested primer 2: | TTCGTGCGCTAGATACGTTG |