| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.137437 |
| Chromosome: | chromosome 10 |
| Location: | 4989068 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455250 | (1 of 6) PF02582 - Uncharacterised ACR, YagE family COG1723 (DUF155) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTGTTGATGGATAGGTGGCGTAAGGGA |
| Internal bar code: | AGCCCAGACGTAGAGGAAATCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 773 |
| LEAP-Seq percent confirming: | 94.8988 |
| LEAP-Seq n confirming: | 11069 |
| LEAP-Seq n nonconfirming: | 595 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGTAGGATGTTCGGGTGC |
| Suggested primer 2: | ACAGTGTATGCGATGCCGTA |