Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.137442 |
Chromosome: | chromosome 6 |
Location: | 3196931 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g275950 | FAP395 | Flagellar Associated Protein 395 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTAGTTTGGGCTAGTGTCTACCCCCACA |
Internal bar code: | CAATATGATGGCACTCAGTTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 826 |
LEAP-Seq percent confirming: | 94.5687 |
LEAP-Seq n confirming: | 4440 |
LEAP-Seq n nonconfirming: | 255 |
LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGACAGAAGCGCTATTCAGG |
Suggested primer 2: | TGAGGGACAGGTGTGTGTGT |