| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.137444 |
| Chromosome: | chromosome 14 |
| Location: | 1131065 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615400 | THB1 | Truncated hemoglobin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGCGGGACCCTCTACTCCGCTCTAAAA |
| Internal bar code: | AGCTCCTCGTCCCTGGCGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 402 |
| LEAP-Seq percent confirming: | 99.2945 |
| LEAP-Seq n confirming: | 563 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGTAAGGAGGCGTGTAA |
| Suggested primer 2: | TCACTTCTGCCACAGCATTC |