| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.137449 |
| Chromosome: | chromosome 14 |
| Location: | 1169877 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615800 | (1 of 3) PTHR31563:SF1 - ION CHANNEL POLLUX-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAACGGACGAACGTCCTAACGCCTTGTT |
| Internal bar code: | GAGGCGGGCCACCCCCCTGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 579 |
| LEAP-Seq percent confirming: | 99.7759 |
| LEAP-Seq n confirming: | 10241 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCAATCTGCTTGTCGGCT |
| Suggested primer 2: | GTCAGCGTGCAAGAAACGTA |