| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137458 |
| Chromosome: | chromosome 12 |
| Location: | 460047 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493550 | THK3 | Thymidine kinase; (1 of 1) PTHR11652//PTHR11652:SF1 - 30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER // 28S RIBOSOMAL PROTEIN S12, MITOCHONDRIAL | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGTGCTGTCGGGTTGGAGGCCGCACGC |
| Internal bar code: | AAAGGCCCGCCCCAACCTTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 105 |
| LEAP-Seq percent confirming: | 46.8085 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGTCATTCCACGACAACA |
| Suggested primer 2: | ATGTATGGTGGCGTGCAGTA |