| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137462 |
| Chromosome: | chromosome_8 |
| Location: | 1119532 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g362550 | VPS37 | Subunit of the ESCRT-I complex | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGCGGTTCGCCCTGCCCTGACCGCTCGCTG |
| Internal bar code: | TTTTGGTTAGCCCCATAAGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 466 |
| LEAP-Seq percent confirming: | 99.0076 |
| LEAP-Seq n confirming: | 4190 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGACAATGAAGTGCTGCC |
| Suggested primer 2: | GGGGCATAAAGCTCATTCAA |