| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.137462 |
| Chromosome: | chromosome_8 |
| Location: | 1119585 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g362550 | VPS37 | Subunit of the ESCRT-I complex | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGACCGCAACCTCTCCCCTGTGAATCTGA |
| Internal bar code: | GGGCGACGCAACGGCGGGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 785 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 621 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGACAATGAAGTGCTGCC |
| Suggested primer 2: | GGGGCATAAAGCTCATTCAA |