Insertion junction: LMJ.RY0402.137477_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.137477
Chromosome:chromosome 8
Location:3912599
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g378250 SMM32 (1 of 1) 2.1.1.85 - Protein-histidine N-methyltransferase / Protein methylase IV; S-adenosyl-L-methionine-dependent methyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACAGTGCGGCTGCATGGCACCGCGCCC
Internal bar code:GCGGGAAACATAGAGTAGGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:951
LEAP-Seq percent confirming:89.2015
LEAP-Seq n confirming:6468
LEAP-Seq n nonconfirming:783
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GACTCTTCATCGTCTTCGCC
Suggested primer 2:CCTAGCGACTCCAGTGATCC