| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.137496 |
| Chromosome: | chromosome 14 |
| Location: | 3569078 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631100 | CEP13,SRR29 | Cysteine endopeptidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGAAGAGATCTTTGCGGGCAGCGGCAGC |
| Internal bar code: | GTTCGGGCAGGTCAGTCTGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 964 |
| LEAP-Seq percent confirming: | 93.5598 |
| LEAP-Seq n confirming: | 16358 |
| LEAP-Seq n nonconfirming: | 1126 |
| LEAP-Seq n unique pos: | 142 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGGCTATGAGGTATGTGT |
| Suggested primer 2: | ACGTCATGCGTTTTGTTGAG |