| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137525 |
| Chromosome: | chromosome 9 |
| Location: | 6151237 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g405600 | SFT1 | (1 of 1) K08505 - protein transport protein SFT1 (SFT1); Intra-Golgi Qc-SNARE protein, Gs15/Sft1 family (Qc.II) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGTGTGGTGCTGGTGGTGCTGGTGGATA |
| Internal bar code: | ACTGAACCGAAAATTGGGACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1256 |
| LEAP-Seq percent confirming: | 99.8653 |
| LEAP-Seq n confirming: | 16306 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGTTCCTCGTTTCAGTG |
| Suggested primer 2: | CAACCACATGCTGTACCTGG |