Insertion junction: LMJ.RY0402.137531_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.137531
Chromosome:chromosome 11
Location:3625315
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g482101 (1 of 2) IPR016024//IPR019399 - Armadillo-type fold // Parkin co-regulated protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTTCTTCTTCCGCCCAGTGCTCCCCGCA
Internal bar code:AATAGGGGCCCATTACAGCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:335
LEAP-Seq percent confirming:80.782
LEAP-Seq n confirming:1219
LEAP-Seq n nonconfirming:290
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:TCGTGTTGACCGGATACAAA
Suggested primer 2:CTCTTGAGCTACTGGGACGG