| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.137546 |
| Chromosome: | chromosome 10 |
| Location: | 2777379 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438950 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCTGATGCATCGCGGCTCGCGCACAGGC |
| Internal bar code: | GTATCCACAGAGCCACAAAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 717 |
| LEAP-Seq percent confirming: | 68.5415 |
| LEAP-Seq n confirming: | 2425 |
| LEAP-Seq n nonconfirming: | 1113 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACGTACCCATCATGTGC |
| Suggested primer 2: | CCACCTGAAATCGGTCAACT |