| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137546 |
| Chromosome: | chromosome 10 |
| Location: | 2777380 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438950 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAGAATGCCAACGCAGGCTAGAAGGAG |
| Internal bar code: | TGTCTGTCCTACAAATGCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 184 |
| LEAP-Seq percent confirming: | 24.1962 |
| LEAP-Seq n confirming: | 2807 |
| LEAP-Seq n nonconfirming: | 8794 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACGTACCCATCATGTGC |
| Suggested primer 2: | CCACCTGAAATCGGTCAACT |