Insertion junction: LMJ.RY0402.137550_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.137550
Chromosome:chromosome 9
Location:788200
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g402051 CLV4 Voltage-gated chloride channel; (1 of 1) PTHR11689//PTHR11689:SF89 - CHLORIDE CHANNEL // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAACACAGTGCTGTAATGCTGGCTTGCGC
Internal bar code:CTATTCAGGGTGCACCCTCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:772
LEAP-Seq percent confirming:99.7166
LEAP-Seq n confirming:2111
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACGCACACACACAC
Suggested primer 2:ACAACATACCCTCTGGTCGC