Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.137551 |
Chromosome: | chromosome_16 |
Location: | 2350849 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
None |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTTCTCCGGAACCCCGATGGGGCACCGTAT |
Internal bar code: | TGGCGGACCACGGCCAAAGGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 275 |
LEAP-Seq percent confirming: | 99.8787 |
LEAP-Seq n confirming: | 1647 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCAACCACTCAAGGAGAAGC |
Suggested primer 2: | ACGACGGATACTGCTGCTCT |