| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137552 |
| Chromosome: | chromosome_8 |
| Location: | 3831255 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g377500 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATTTGTGTGCGTGCGTGTGGCGTGTTCCGT |
| Internal bar code: | TTTAATGTTTTTTCATCGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 67 |
| LEAP-Seq percent confirming: | 8.13636 |
| LEAP-Seq n confirming: | 179 |
| LEAP-Seq n nonconfirming: | 2021 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCTACCTTGTAGTCGTGC |
| Suggested primer 2: | ACCTAATCTTTGCACCCACG |