| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137576 |
| Chromosome: | chromosome 12 |
| Location: | 1116293 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490451 | (1 of 16) IPR011044 - Quinoprotein amine dehydrogenase, beta chain-like | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGACGATGGCTTAGCACGGGAGAGACTC |
| Internal bar code: | TGGTAGACGGCTAGACTAGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 99.4917 |
| LEAP-Seq n confirming: | 2936 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTGCAATGAACTACCCCC |
| Suggested primer 2: | ACTCAGCAGCAGCAATAGCA |