| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.137589 |
| Chromosome: | chromosome_2 |
| Location: | 1248160 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g082550 | ZEP,ZEP1 | Zeaxanthin epoxidase | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACCCACAAATGAACACCATCCCCTAGTCAT |
| Internal bar code: | ACCAGGCAAACCATGCCGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 839 |
| LEAP-Seq percent confirming: | 99.8454 |
| LEAP-Seq n confirming: | 7102 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGACAAGCAGTGAAGGAA |
| Suggested primer 2: | CGGCAAGGATGAAATGTTTT |