Insertion junction: LMJ.RY0402.137589_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.137589
Chromosome:chromosome 2
Location:1248160
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g082550 ZEP,ZEP1 Zeaxanthin epoxidase 1; (1 of 1) K09838 - zeaxanthin epoxidase (ZEP, ABA1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCACAAATGAACACCATCCCCTAGTCAT
Internal bar code:ACCAGGCAAACCATGCCGGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:839
LEAP-Seq percent confirming:99.8454
LEAP-Seq n confirming:7102
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGACAAGCAGTGAAGGAA
Suggested primer 2:CGGCAAGGATGAAATGTTTT