Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.137609 |
Chromosome: | chromosome_1 |
Location: | 7185067 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
None |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGTGGTTTGGCCGATCCGGCGCAGGGGGG |
Internal bar code: | CTCGTAATGCCGGCTCGATTTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 278 |
LEAP-Seq percent confirming: | 98.8621 |
LEAP-Seq n confirming: | 2867 |
LEAP-Seq n nonconfirming: | 33 |
LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CATCCACCCTCTACCCTTCA |
Suggested primer 2: | AACATTGGCGTTTCTTGGTC |