| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.137609 |
| Chromosome: | chromosome_9 |
| Location: | 6798850 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g409600 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGATGGCCCGCGCCGTACTTGGCGCAGTA |
| Internal bar code: | CGCCAGCAAAGGAGGCTAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 864 |
| LEAP-Seq percent confirming: | 99.2789 |
| LEAP-Seq n confirming: | 218756 |
| LEAP-Seq n nonconfirming: | 1589 |
| LEAP-Seq n unique pos: | 373 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACAAGAGGAGCAAACACG |
| Suggested primer 2: | GCACTTGACAGGCATTCTCA |