| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137611 |
| Chromosome: | chromosome 16 |
| Location: | 1871530 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g655600 | (1 of 4) PF16016 - Domain of unknown function (DUF4782) (DUF4782) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCCGCCCTGCAGCGAGGAGCGTCTTTCC |
| Internal bar code: | TTTATGATCTGTCAAACATAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 235 |
| LEAP-Seq percent confirming: | 82.2727 |
| LEAP-Seq n confirming: | 362 |
| LEAP-Seq n nonconfirming: | 78 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCCAAAGTGGCCTTGTTA |
| Suggested primer 2: | AGTCACCCAGGGGAAAGAGT |