| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137631 |
| Chromosome: | chromosome 10 |
| Location: | 1578521 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429250 | (1 of 3) 2.7.11.25//2.7.12.1 - Mitogen-activated protein kinase kinase kinase / MLTK // Dual-specificity kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAATCAAAAACACACGCAGTTGTCGTGTT |
| Internal bar code: | GGCGGATGTGCTGTTGTTATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 388 |
| LEAP-Seq percent confirming: | 99.5743 |
| LEAP-Seq n confirming: | 2573 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACTGTGAAAGGCAGAAGC |
| Suggested primer 2: | ACGGTCTGGTGTTACAAGCC |