| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.137635 |
| Chromosome: | chromosome 6 |
| Location: | 6028123 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g289400 | PDE11 | (1 of 20) 3.1.4.17 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGAGCTCAGTCATGGAGGGGCTCGTTCC |
| Internal bar code: | GCGTTTCCTGGGTCGGATGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 943 |
| LEAP-Seq percent confirming: | 92.7073 |
| LEAP-Seq n confirming: | 7246 |
| LEAP-Seq n nonconfirming: | 570 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACTAGCAGCGGAATGAT |
| Suggested primer 2: | AACGCCAATCAGGCATTATC |