Insertion junction: LMJ.RY0402.137635_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.137635
Chromosome:chromosome 6
Location:6028740
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g289400 PDE11 (1 of 20) 3.1.4.17 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGAGCCAAGGTACGTCCCAGGATGCAAGG
Internal bar code:GAGCTGTCCCATGCAACACATC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0659015
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:18197
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTAAACCCACATGCGAAC
Suggested primer 2:TAATGCTATGCTCCTGCGTG