| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137638 |
| Chromosome: | chromosome 8 |
| Location: | 977454 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361984 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGCGCGTGTGCAGGTGGCAGGAGAGGA |
| Internal bar code: | GGCTGGAATAATTCTCCCGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 859 |
| LEAP-Seq percent confirming: | 99.5454 |
| LEAP-Seq n confirming: | 30000 |
| LEAP-Seq n nonconfirming: | 137 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTGGAGTGCTGGAAATC |
| Suggested primer 2: | GTGGCGTTCATATACCGCTT |