| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137648 |
| Chromosome: | chromosome 14 |
| Location: | 1901605 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g620800 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAAACTAGAGTAGCCCGGCTGCTCACCCG |
| Internal bar code: | GGTAGGAGGTTACCCTCGCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 529 |
| LEAP-Seq percent confirming: | 99.6195 |
| LEAP-Seq n confirming: | 8639 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGTGCGTGTGTGTGT |
| Suggested primer 2: | GTCTGCTGGAGAAGCTTTGG |