| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137651 |
| Chromosome: | chromosome 1 |
| Location: | 1839530 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010000 | MAPK8 | Mitogen-Activated Protein Kinase; (1 of 1) K14512 - mitogen-activated protein kinase 6 (MPK6) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGGGCTTCAAATCTCTGTGCAGGATGT |
| Internal bar code: | CTCTCATCAGCCATCTAACTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 709 |
| LEAP-Seq percent confirming: | 98.8715 |
| LEAP-Seq n confirming: | 1139 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTCCCTACCTCCACTACA |
| Suggested primer 2: | ACCTTGGTGATGAGGGACAG |